Laboratory - Image illustration

The present collection of micro-photographs aims the site visitor to gain experience on how the magnificent microcosm of cells is depicted in the laboratory under microscope. It represents a part of an extensive collection of micro-photographs taken by using a phase-contrast inverted microscope, specific for cell culture monitoring, and applying the Hoffman Modulation Contrast, HMC technique.

Section 1:

1a. Human fibroblasts as cellular entities: Morphology - staining - lipogenesis - colonies (CFU-F assay)

1b. Formation of a human fibroblast layer: Formation stages - staining - fibroblast interference - lipogenesis - Confluent stroma - The non-confluent layer of the marrow in non-confluent stroma (fibromyalgia)

1c. Fat cells: Morphology - colony formation - staining - aging - physiological comparison, with acute leukemia marrow fat cells.

Section 2: The CAM method is a two-stage LTBMC assessing the potential of hematopoietic stem cells.

2a. M2-10B4 mouse hybrid fibroblast line: Morphology - staining - lipogenesis - colonies (CFU-F assay).

2b. Stroma layer formation with the M2-10B4 cell line: Formation stages - staining - fibroblast interference - lipogenesis.

2c. Cobblestone Areas (CAs): Formation - maturation - mixed - megakaryocytes in CaS - CA from normal marrow - CA from myelodysplastic syndrome.

2d. Colony Forming Unit (CFU-progenitor cells): CFU-E, BFU-E / mature / primitive), CFU-GM, CFU-M, CFU-Eos, CFU-Meg, CFU-GEMM.

Section 3:

3a. Contamination of cell cultures.

3b. Detachment of fibroblast layer.

3c. Count nucleated cells with the Neubauer hemocytometer (glass haemocytometer).

3d. Cells from the supernatant of a h-LTBMC (cytospin preparations).

Section 4:

4a. Cell culture of 5637 human cell line (cancerous bladder epithelium).

4b. Cell culture of the human osteoblastic line SaOS-2 (osteosarcoma).

Image illustration: